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Discover the Power of DNBSEQ™ Technology
A Revolution in DNA Sequencing
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The DNBSEQ™ Advantage
Our DNBSEQ™ technology offers several key advantages:
Increased Accuracy: DNBSEQ™ technology combines the low error accumulation of DNA Nanoballs (DNB) with the high signal density of array chips, resulting in significantly improved detection accuracy, especially for Whole Genome Sequencing (WGS) and Whole Exome Sequencing (WES).
Efficient Preparation and Amplification: The DNBSEQ™ process includes efficient DNA single strand circularization and DNB making, using rolling circle replication (RCR). RCR reduces error introduced during amplification, leading to greatly improved sequencing accuracy.
Optimized Utilization and High Throughput: The patterned array and DNB loading ensure high sequencing accuracy, optimal chip utilization, and efficient reagent usage. This results in high throughput and cost-effective sequencing.
Advanced cPAS Technology: The Combinatorial Probe-Anchor Synthesis (cPAS) technology incorporated in DNBSEQ™ allows for high-speed, high-accuracy base calling, significantly improving the sequencing reaction time.
Proprietary Base Calling Algorithm: MGI's proprietary Sub-pixel Registration algorithm and GPU accelerated algorithm enable image intensity extraction at the sub-pixel level, greatly improving base call accuracy and dramatically increasing data processing speed.
How DNBSEQ™ Works
DNA Preparation
Our process starts with double-stranded DNA, which we heat to separate into single strands. A 'splint oligonucleotide' binds to these strands, forming a 'nicked circle.' This circle is then sealed by DNA ligase, creating a continuous single-stranded circle. This precise method ensures reliable DNA sequencing results.
DNB Making
DNA Nanoballs (DNBs) are created through Rolling Circle Replication (RCR), producing 300-500 copies from a single DNA template. This method, featuring high-fidelity DNA polymerase, minimizes replication errors, enhancing the accuracy of our DNBSEQ sequencing platform.
DNB Loading
DNBs, negatively charged, adhere to the positively charged flow cell surface, thanks to their phosphate backbone. Special buffers maintain signal integrity throughout sequencing, ensuring flow cell spot hosts only one DNB for optimal sequencing efficiency.
cPAS
In cPAS, sequencing begins with primers binding to DNA Nanoballs (DNBs), followed by the addition of fluorescent dNTP probes. After imaging and signal conversion to digital data, a regeneration reagent prepares DNBs for the next cycle. Our advanced sequencing polymerase, selected from thousands of mutants, speeds up the sequencing reaction, enhancing efficiency and accuracy.
2nd Strand Preparation
After the first strand is sequenced, we synthesize the second strand using special primers and a polymerase with strand displacement activity. This process creates a stronger signal for the second strand, improving sequencing accuracy through optimized chemistry.
Base Calling Algorithm
Our base-calling algorithm uses signal intensity to determine base calls and their quality, aligning with PHRED-33 standard. Enhanced by a proprietary Sub-pixel Registration algorithm, it achieves precise base call determination at a sub-pixel level, ensuring industry-leading speed and accuracy in data processing