Revolutionize Your Mycobacterium Tuberculosis Research with MGI's Cutting-Edge ATOPLEX & MTB-Explorer Software
Next Generation Sequencing has provided insights into the genetic diversity of Mycobacterium Tuberculosis, which are crucial for understanding the evolution and transmission of the disease, including the identification of drug-resistant strains, enabling rapid and accurate tailoring of treatment.
MGI has developed an ATOPlex Mycobacterium Tuberculosis targeted sequencing sample to report workflow, providing full and complete MTB identification and drug resistance analysis. Based on MGI’s ATOPlex multiplex PCR library preparation platform, 2-step amplification in one tube is needed to achieve target area amplification and library construction, supporting direct detection of nucleic acid extracted from sputum and lavage fluid samples.
Combined with MGI’s MTB-Explorer Software, provides identification of 174 types of mycobacteria, MTBC identification, spoligotyping, and evolutionary traceability analysis. Specifically for drug resistance, the software covers a total of 7000+ mutations, including all of the 1200+ first-and second-group drug resistance associated mutations associated with the “Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance" released by WHO for annotation.
MGI's ATOPlex Mycobacterium Tuberculosis targeted sequencing workflow Sample to report in as fast as 24h
Sample preperation
~7-8h
ATOPlex MTB Library Preperation Set
DNBSEQ OneStep Make DNB Reagent Kit V4.0 (Dua Barcode)
1
Sequencing
~12-20h
High-throughput Sequencing Set
DNBSEQ-G99 SM FCL PE150 12h
DNBSEQ-E25 FCL PE150 20h
2
Analysis
~2-6h
MTB-Explorer Software
3
ATOPlex Mycobacterium Tuberculosis Targeted Sequencing Package
Precisely analyze and annotate drug resistance and explore with MTB-Explorer Software (ATOPLex)
MTB-Explorer Software (ATOPLex)
V1.0.0
Summary
Data
Quality control
Clean data
MTBC species
reference
Species identification
Negative/Positive
judgement
Negative
report
Positive
M.tuberculosis
reference
(H37Rv)
Alignment
Lineage &
Spoligotype
Lineage &
Spoligotype
results
Variant Calling
Consensus
sequence
Evolutionary analysis (Tree)
Evolutionary analysis (Tree)
Annotation
MTB drug resistance database
Drug Resistance
Prediction results
Mycobacterium Tuberculosis Next Generation Sequencing DNBSEQ-G99/E25
A powerful tool for Mycobacterium Tuberculosis analysis
Library Preparation
Sequencing
Data Analysis
![](https://framerusercontent.com/images/WSu1ZO7vLv7cqPmAjUsAsn1a58M.webp)
ATOPlex MTB Library Preparation Set
Designed specifically for MTBC identification and MTB drug resistance gene detection
![](https://framerusercontent.com/images/WSu1ZO7vLv7cqPmAjUsAsn1a58M.webp)
DNBSEQ OneStep Make DNB Reagent Kit V4.0 (Dua Barcode)
Simplified sequencing process: Direct access to DNB samples.
![TB Reagent Kits and Flowcell](https://framerusercontent.com/images/7XOnjBp1MRWCYVYjtl59dcKBL8U.png)