Can Next-generation Sequencing accelerate mRNA vaccine development?

Oct 10, 2023

Share article
Dr. Weilin Liu

Dr. Weilin Liu - Field Application Scientist at MGI

Can Next-generation Sequencing accelerate mRNA vaccine development?
Can Next-generation Sequencing accelerate mRNA vaccine development?
Can Next-generation Sequencing accelerate mRNA vaccine development?

Katalin Karikó and Drew Weissman have been granted the 2023 Nobel prize in Physiology or Medicine for their subtle but significant discoveries concerning nucleoside base modifications (Fig1. ), namely replacement of uridine by N1-methylpseudouridine (m1Ψ), which serves as the cornerstone to make mRNA delivery non-immunogenic and to enable the development of mRNA vaccines against COVID-19 with the unprecedented effectiveness and speed, saving millions of lives and ending not only the pandemic of a disease but also the catastrophic health-care burden globally.

Currently, many new mRNA-based therapies have been vigorously developed for a wide variety of diseases, including other infectious pathogens, cancer and auto-immune diseases. In the meanwhile, the concerns about its safety and potential side-effects have been undoubtfully arisen as happened to other new treatments. Though diverse techniques, such as RT-qPCR, capillary gel electrophoresis, IP-RP-HPLC and immunoblotting have been implemented in the quality analysis of mRNA vaccines, those methods are tedious, expensive and lack of sensitivity in detection essential mRNA features. Consequently, a demand on a robust and combinatory analytical method is urgently needed to evaluate mRNA vaccines integrity, efficiency, and more importantly to monitor contaminants and off-target effect to address those safety and specificity concerns. In the last few years, Next-generation sequencing technology has been advanced with a rapid pace on multiple aspects, including improved sequencing throughput capacity, speed, accuracy, significant reduction of cost and long-read development, promoting its various applications in both research and clinical diagnosis. Can next-generation sequencing assist the development and validation of mRNA vaccines and therapies??


An RNA-sequencing study published recently in Nature communication combined and evaluated both short- and long-read sequencing for mRNA vaccine and therapies analysis. The study proposed a streamlined method, VAX-seq workflow (Gunter et al., 2023) followed by an integrated bioinformatic tool called Mana to gain a comprehensive assessment on those mRNA key attributes, including sequence integrity, 3’-end poly(A) tail length and DNA/RNA contaminants (Fig. 2). Moreover, VAX-seq also provides a solution in the detection of off-target RNAs, which are generated during in vitro transcription as truncated, readthrough or anti-sense form of RNAs triggering innate immune response. Both long- and short-read sequencing revealed the similar on- and off-target RNAs percentage.

About MGI

MGI Tech Co., Ltd. (MGI) is at the forefront of global innovation, actively contributing to life science through intelligent innovation. With a presence in over 100 countries and a customer base of 2600+, MGI's cutting-edge technology has been instrumental in the development of 736+ user patents, facilitating the creation of over 150 petabytes of data. The company's extensive portfolio includes sequencing instruments, automation instruments, reagents, and related products that cater to various sectors such as life science research, agriculture, precision medicine, and healthcare.

MGI is dedicated to advancing life science tools for the healthcare of the future. As of December 2021, the company's global presence has expanded to more than 100 countries and regions, serving over 1,300 international clients. With a workforce of over 2,900 professionals worldwide, including 5 centers for research and development and production facilities in Europe, MGI is committed to fostering innovation. Approximately 35% of MGI's employees are engaged in R&D, underscoring the company's focus on pioneering advancements in the field.

The impact of MGI's work is further evidenced by the publication of over 6,800 papers in prestigious scientific journals, showcasing the significant contribution of MGI's technology to the scientific community and beyond.

For more information about MGI and its contributions to life science and healthcare, please visit the MGI website or connect with us on Twitter, LinkedIn, or YouTube.

MRNA VACCINE DEVELOPMENT

NEXT-GENERATION SEQUENCING

VAX-SEQ WORKFLOW

NOBEL PRIZE IN MEDICINE

Share this article :

Share

More Science

Smart-seq3Xpress: a scalable, high-sensitivity and low-cost Single cell Full-length RNA-seq methodology.
Smart-seq3Xpress: a scalable, high-sensitivity and low-cost Single cell Full-length RNA-seq methodology.
Smart-seq3Xpress: a scalable, high-sensitivity and low-cost Single cell Full-length RNA-seq methodology.
Smart-seq3Xpress: a scalable, high-sensitivity and low-cost Single cell Full-length RNA-seq methodology.

Mar 5, 2024

Smart-seq3Xpress: a scalable, high-sensitivity and low-cost Single cell Full-length RNA-seq methodology.

Revolutionizing single-cell RNA-seq, SMART-seq3Xpress offers unparalleled sensitivity and cost-efficiency with full-length coverage.

Single-cell Spatial transcriptomics (sc-StereoSeq), opening a new era in plants and crops biology.
Single-cell Spatial transcriptomics (sc-StereoSeq), opening a new era in plants and crops biology.
Single-cell Spatial transcriptomics (sc-StereoSeq), opening a new era in plants and crops biology.
Single-cell Spatial transcriptomics (sc-StereoSeq), opening a new era in plants and crops biology.

Jan 10, 2024

Single-cell Spatial transcriptomics (sc-StereoSeq), opening a new era in plants and crops biology.

Explore how sc-StereoSeq is transforming our understanding of plant biology, from crop production to plant stress responses, marking a new era in spatial transcriptomics.

A Complete Human Y to ask more "Why" in biology
A Complete Human Y to ask more "Why" in biology
A Complete Human Y to ask more "Why" in biology
A Complete Human Y to ask more "Why" in biology

Sep 1, 2023

A Complete Human Y to ask more "Why" in biology

Unlock the mysteries of the human Y chromosome with MGI's cutting-edge findings. Learn how the T2T consortium's study corrects GRCh38 errors, reveals 30Mb+ new sequence data, and advances our understanding of gene structures and their implications in the Omic-verse.

Join our newsletter to stay up to date on features and releases.

I have read and understood MGI’s Privacy Policy, and I consent to the collection and processing of my personal data for handling, responding to my contact, receiving your newsletter as well as promotion and marketing activities.

Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.

Join our newsletter to stay up to date on features and releases.

I have read and understood MGI’s Privacy Policy, and I consent to the collection and processing of my personal data for handling, responding to my contact, receiving your newsletter as well as promotion and marketing activities.

Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.

Join our newsletter to stay up to date on features and releases.

I have read and understood MGI’s Privacy Policy, and I consent to the collection and processing of my personal data for handling, responding to my contact, receiving your newsletter as well as promotion and marketing activities.

Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.